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Starting life as an ABI 3900, the Biolytic 3900 is an upgrade of the original ABI 3900 that comes packed with new hardware and reimagined software features as well as a new computer and monitor running the latest Windows operating system. The new software is built for Windows 8 and features flexible protocol construction similar to the world renowned Dr. Oligo Synthesizer. The software supports truly independent protocol operation on each bank of 12 oligos while providing the user with real-time feedback during each synthesis run.

With over two decades of applied experience in the field of oligonucleotide synthesis automation, Biolytic is able to bring you a 3900 upgrade that dramatically improves its performance, adds hardware features and comes with a completely new software package, written from the ground up.

Biolytic 3900 Front Panel
Front Panel

Biolytic 3900

The patent pending Biolytic 3900 greatly reduces cycle time due to the use of 4 drain stations rather than the original 2. The chamber volume is much lower, requiring less time to pressurize and vent.

A spacer is placed on top of the column rotor, speeding up the pressurization and venting process and reducing gas consumption.

The Biolytic 3900 adds a mechanism to move reagents slowly through the columns creating maximum reaction site efficiency. This allows the use of less reagent while achieving a high reaction efficiency. Most oligos are synthesized using CPG or polystyrene support columns.

The reaction sites within the solid supports are in tight spaces (such as CPG pores), requiring time for reagent to diffuse to the reaction sites. The ability to precisely control reagent flow through the columns allows more efficient diffusion to reaction sites, resulting in higher reaction efficiency even with the use of smaller volumes of reagents.

Biolytic 3900 Bank Vent & Reagent Spill Trough
Bank Vent & Reagent Spill Trough

Biolytic 3900

The patent pending Biolytic 3900 also allows finely tuned control of reaction chamber pressure above and below synthesis columns, preventing liquid from flowing backwards up through columns and eliminating the risk that back flowing reagents may contaminate adjacent syntheses.

The Chamber Flood Damage Control System redirects chamber flood reagents to waste. In the unlikely event of a reagent overflow, excess liquid in the reaction chamber is directed to a waste trough away from the motion control assembly. This protects the motion control assembly from chemical damage, preventing the need for potentially costly and time-consuming repairs.

Truly independent protocol operation on each bank of 12 oligos. The pressure below each bank of 12 synthesis columns is controlled independently, allowing the simultaneous use of up to 4 different protocols in a single synthesis.

Allows use of up to 10 specials. The amidite delivery is highly customizable, allowing any amidite nozzle to dispense to any column position, permitting the use of up to 10 special amidites in addition to the 4 standard amidites used.

Ability to simultaneously use 2 different Oxidizer reagents.The software package allows the use of more than one oxidizer, and permits the configuration of each amidite to automatically select the correct oxidizer to be used in each step of the synthesis. Among other applications, this allows oxidation and sulfurization to be performed in the same synthesis.

Flexible protocol construction. There is no need to use Excel to edit and create protocols. Biolytic's protocol editor uses familiar Drag-and-Drop to edit and create protocols. Select from a range of standard protocols supplied with the software, or finely tune your protocols to meet you individual synthesis needs.

More Features

  • Increased Performance
  • Increased Efficiency (Reduced Reagent and Gas Consumption)
  • Completely eliminates reverse flow cross contamination
  • Reduces errors rates in oligos used for gene construction
  • Allows use of up to 10 specials
  • Ability to simultaneously use 2 different Oxidizer reagents
  • Fine control of reagent flow rate to minimize reagent consumption
  • Truly independent protocol operation on each bank of 12 oligos
  • Biolytic owned and developed software and hardware
  • Customization of hardware & software available upon request


  • User Control New computer & monitor running latest Windows
  • Valve Control Intelligent valve control boards
  • Motion Control Completely closed loop intelligent motion control
  • Reagent Spill Containment Redirected in chamber bottom away from motor system
  • Pneumatics Requires 60 Psi (0.413 Mpa) to 80 psi (0.551 Mpa)
  • Power 100 to 240 VAC, 50 / 60 Hz, 800 VA
  • Manufactured Made in the U.S.A.



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