Molecular Trap / Reagent Trap Packs / Molecular Sieve

Biolytic Lab Performance. Inc. offers Molecular Traps that are designed to remove unwanted moisture from various reagents. These Molecular Traps are offered in two varieties.

There is a Molecular Trap designed to remove moisture from solvents. It is very popular for drying reagents used in Oligo Synthesis and for maintaining low moisture in these reagent if the reagents are left on a synthesizer for extended periods of time.

There is also a Molecular Trap that is designed to remove moisture and amines from DMF and NMP for peptide synthesis.

Molecular Traps, Stability Period

The Molecular Traps are useful in various solvents and have different stability periods depending on the solvent they are placed in. The following is a table that gives a listing of the solvents that the Molecular Traps are effective in. It also breaks the solvents into two categories.

Category one contains reagents that the Molecular Traps are stable in for longer than one month.

Category two contains reagents that the Molecular Traps are stable in for up to one month.

General Solvent Exposure Table

Category -1

Solvents Suitable for Extended Exposure

Category - 2

Solvents Suitable for Exposure up to One Month

Acetone

Chloroform

Acetonitrile

Methylene Chloride

Benzene

Tetrahydrofuran

Diethyl ether

Toluene

Dimethylformamide (DMF)

Dioaxne

Ethyl Acetate

Ethanol (Anhydrous)

Hexane / Heptane

Methanol (Anhydrous)

Methylethyl Ketone (MEK)

N-Methylpyrolidinone (NMP)

Molecular Traps in Oligo Synthesis

Molecular traps are used in Oligo Synthesis reagents to maintain low water in water sensitive reagents. The Molecular Traps can be used for an extended period of time in Oligo Synthesis Reagents without degradation of the packing material or the casing.

The determining factor for how long to use a Molecular Trap on oligo synthesis reagents before changing to a new trap is really dependent on the amount of moisture the molecular trap has been exposed to. The wetter the solvents, the shorter the life of the molecular trap.

As a molecular trap absorbs moisture from the solvent its moisture absorbing capacity diminishes. At some point the molecular trap will become saturated and will no longer absorb moisture but it may release moisture into the solvent if the solvent is very dry to begin with. Therefore it is important to not use molecular traps once they have become saturated with water.

There is no visual indication of the moisture content of a molecular trap.

The user must judge the useful life based on the initial water content of the reagent and the humidity in the air.

A relatively safe useful life time is 4 weeks or 4 bottles of solvent which ever comes first.

Molecular Trap Pack Reagent Water ppm Graph

Oligo Synthesis Reagent Table (Click the trap size below to order)

Reagent

Bottle Size, ml

Trap Size

Acetonitrile

4000 ml

20 gram

Acetonitrile

2000 ml

10 gram

Acetonitrile 1000 ml 10 gram  

Acetonitrile

500 ml

5 gram

Activator 1000 ml 10 gram  

Activator

500 ml

5 gram

Amidite *

20 ml

1 gram

Amidite *

60 ml

2 gram

Amidite *

100 ml

2 gram

* Amidites are purchased in dry powder form. Upon dissolving the amidite in Anhydrous Acetonitrile, the molecular trap can be added.

Moisture Absorbing Action

Molecular Traps can dry solvents that start out slightly wet however it is recommended that if the solvent contains more than 0.1% water 2 molecular traps be used per bottle.

Reducing the moisture content of a reagent that is wet takes time. The user should add the Molecular Trap and wait 24 to 48 hours before using it to make sure that the water content has been reduced to a useable level (< 50 ppm).

Molecular Traps can also be used to maintain a low water content in solvents. This process is ongoing and takes place at the same rate that moisture is absorbed by the solvent if absorption is due to normal exposure while the reagent is attached to an instrument.

 

Molecular Traps in Peptide Synthesis

Moisture & Amine Removing Molecular Traps

These molecular traps are designed to be used in DMF and NMP for Peptide Synthesis. DMF is very hygroscopic. Moisture in DMF degrades DMF into carbon monoxide and dimethylamine (DMA). DMA degrades fmoc amino acids and therefore is bad for fmoc peptide synthesis.

Molecular Traps have been shown to reduce moisture in DMF and NMP as well as remove DMA from DMF.

Peptide Synthesis Reagent Table (Click the trap size below to order)

Reagent

Bottle Size, ml

Trap Size

 

DMF / NMP

4000 ml

20 gram

DMF / NMP

2000 ml

12 gram

DMF / NMP

100 ml

6 gram

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